Yesterday I was once again allowed to "play" with the confocal microscope; actually this was the second of my training sessions (for a total of 4 hours), so now I'm kind of an expert (not really) and allowed to use it on my own. Certainly I'm not close to being a know-it-all with the confocal scope, but it is nice to be able to make the judgment between just a pretty image and an accurate representation of what is on the microscope slide. I was quite surprised to learn that sometimes the two things don't go hand in hand. Today the first sample that I worked with came from Zeiss, and thus all of the names on the microscope slides were in German. While my instructor had never heard of a Maiglöckchen, if you have ever been to Germany in the spring, then you certainly have seen lily of the valley flowers. Though I have to admit that I don't know much about plant biology, the images are of the rhizome, which is the underground stem of the plant. The dyes used were a rhodamine derivative and fluorescein isothiocyanate (FITC) .
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